Contents of mangiferin and genkwanin were detected by High Performance Liquid Chromatography (HPLC) to explore the dynamic changes of active chemical components from different growing year and different sampling season in Aquilaria sinensis leaves and determine the appropriate growing year and season for sampling. It is disclosed that, growing year was an important influence on content of mangiferin, the appropriate growing year for mangiferin was two to three year and the highest content, 2.31% of mangiferin was in two-year A. sinensis leaves, the appropriate growing year for genkwanin was one to two year and the highest content, 0.23% of genkwanin was in one-year A. sinensis leaves; while the appropriate sampling season for mangiferin was Autumn and the appropriate sampling season for genkwanin was Spring.