In order to undersd and the distribution features of SPAD value at different part of blade and different leaf order of sugarbeet, both pot and field experiment with different sugarbet genotypes were conducted by using SPAD- 502 chlorophyll meter. And the correlations between leaf SPAD value of different development stages and leaf chlorophyll content or the plant nitrogen content were analyzed in different genotypes of sugarbeet. The results were as follow: The leaves's SPAD values of sugarbeet were different; the blade tip of the highest leaf in sugarbeet plant should be taken as the optimum measured position of SPAD value. With the addition of nitrogen applying rate, the SPAD values increased. There were significant relationships between SPAD values and chlorophyll content, plant nitrogen. So the SPAD values were suitable for rapid diagnosis of nitrogen nutrition and used to monitor the development and photosynthetic efficiency in sugarbeet production.