The fatty acids composition analysis was carried out by Gas Chromatography (GC) to determine the mass fraction of methyl esters of individual fatty acids to their sum. Based on our study, the seeds of all 30 varieties from the nursery of competitive variety trials had similar (but not identical) fatty acid compositions and contained low amounts 12-14% of saturated fatty acids and high amounts 82-86% of unsaturated fatty acids. It has been confirmed that the unsaturated fatty acids were pre-dominant in all varieties. Received results confirm the presence of unsaturated oleic (C18:1) and polyunsaturated linoleic (C18:2) and linolenic (C18:3) acids in all soybean seed oils. Linoleic acid was the main fatty acid in the present study: Linoleic acid≥Oleic acid ≥Palmitic acid ≥ Linolenic acid ≥ Stearic acid Screening on the content of polyunsaturated fatty acids (PUFA) in the soybean oil demonstrated that all 30 varieties so far were characterized by a high content of PUFA nearly 60%. The lowest content of PUFA of about 57% was seen in standard Misula and vrs. V10/1012, ZR-30, B44/21. The saturated/unsaturated acid ratio was generally low. The lowest and highest are 0.15 (varieties ZPR-13, V21/311 and B40/623) and 0.18 (varieties Misulast., А16/145, А9/363, А9-562, B-47/53, V5/221), respectively.