- 期刊
- OpenAccess
The Promotion Effect of Polysaccharide Isolated from Fresh tremella on the Growth of bifidobacteria
摘要
The current research aimed to evaluate the growth promotion effect of polysaccharide extracted from the fresh tremella (TOS) on bifidobacteria. Response surface methodology was firstly used to optimize the extraction parameters. The optimum extraction conditions, defined as those resulting in a maximum TOS yield, were liquid (water) material ratio 15 mL/g, temperature 80°C and extraction time 3 h, achieving high polysaccharide extracts yield of 26.41%. The content of carbohydrate and protein from TOS were determined as 0.38±0.02 g/g and 41.70±0.17 mg/g by phenol-sulphuric acid method and Coomassie bluemethod, respectively. The effect of TOS on the growth of bifidobacterium adolescentis in vitro was investigated by substituting glucose in the original culture medium with TOS. The results showed that TOS significantly increased the proliferation of bifidobacterium compared to glucose at all tested concentration ranging from 0.5 to 16 g/L, while TOS produced a comparable effect on bifidobacterium growth to the positive control (Fructooligosaccharides (FOS)) at higher concentration ranging from 4 to 16 g/L. In conclusion, TOS demonstrated a strong propagative effect on Bifidobacterium as carbon source.