Determination of Bicarbonate in Fermentation of Cyanobacterial by Non-Suppress Ion Chromatography

Aihua Xu ； Xin Wang ； Yun Fa ； Yinghui Liu ； Fangfang Li ； Hailan Huang ； Zonghua Wang

《Advance Journal of Food Science and Technology》 13卷1期 (2017/01) Pp. 7-10

https://doi.org/10.19026/ajfst.13.3410

摘要

Separation of Bicarbonate had been investi gated using a non-suppress cation chromatography and conductivity detection. Analysis was performed on an Optimix C18/SCX Cation-exchange column coupled to a conductivity detector, with an injection volume of 25 μL and a flow rate of 0.2 mL/min. The leacheate was fermentation of cyanobacterial apart from sodium bicarbonate. Under the optimized conditions, the linear relationship of Bicarbonate was 0.99946 in the range between 01 and 500 mg/L. The relative standard derivations of Bicarbonate less than 1.0% (n = 8). The recoveries of Bicarbonate in the samples from 100.15% to 111.10% were obtained. The Limit Of Detection (LOD) was 0.5 mg/L. The result showed that the method was convenient and accurate for the determination of Bicarbonate in Cyanobacterial fermentation.

關鍵字

Anion exchange chromatography ； cyanobacteria ； glucosylglycerol ； pulsed amperometric detection ； sucrose