The jujube cultivar 'Qiyuexian' was chosen as object in this study. Effects of different concentrations of brassinolide (BL) on its characteristic parameters of light/CO_2 response curve were investigated. The results indicated that the mentioned parameters were significantly affected by the concentrations of BL. The Light Saturation Point (LSP) was significantly increased by 17.42% after being treated by 0.1 mg/L BL. After spraying 0.2 mg/L BL, the maximum net photosynthetic rate (P_(nmax)), apparent quantum efficiency (AQY), LSP, maximum carboxylation rate (V_(cmax)), maximum rate of photosynthetic electron transport (J_(max)) and Triose Phosphate Utilization rate (TPU) were significantly increased by 21.14, 20.26, 21.95, 21.14, 20.26 and 21.95%, respectively. The AQY, LCP, LSP, V_(cmax) and J_(max) were significantly increased by 12.55, 60.75, 19.00, 20.96 and 20.06% respectively after being treated by 0.3 mg/L BL, while the LCP and LSP were significantly increased by 65.53% and 25.79% respectively after being treated by 0.4 mg/L BL. In conclusion, 0.2 mg/L BL treatment could significantly accelerate the overall photosynthetic ability of "Qiyuexian" jujube, promoting its accumulation of assimilative products, which is beneficial to increase the production and quality of jujube fruits.