Brucellosis is endemic in Pakistan. Keeping in view the importance of the disease and non availability of scientific information, a study was designed to carry out the sero-prevalence of brucellosis using an indirect ELISA and Rose Bengal Precipitation Test (RBPT). Serum samples were collected from three types of farm categories having different husbandry practices. (1) Government livestock farms located in Punjab province (2) Privately owned farms located in out skirts of Lahore and (3) Dairy cattle colony (Gawala colony) located in Lahore. I-ELISA detected sero-conversion in 15.2% buffaloes and 9% cattle, whereas, by using RBPT it was 8 and 6.5% in buffaloes and cattle, respectively. In cattle, more abortions were recorded at private farms (17.86%) followed by gawala colonies (11.61%) and government livestock farms (8.92%). Also, I-ELISA detected sero-conversion against brucellosis in 13.2% female and 1.3% male animals, while by using RBPT it was 7.9% in female and 1.6% in male animals, respectively.