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In Vitro Evaluation of Extracts of Solanum torvum (Solanaceae) Fruits for Anti-Trichomonal Activity
並列摘要
Trichomonasis is curable when treated. However there is increasing incidence of resistance to therapeutic drugs. Solanum torvum has several ethnomedicinal applications and therefore a potential source of antiparasitic drugs. The objective of this study was to assess the therapeutic potentials of extracts of fruits of Solanum torvum against Trichomonas gallinarum. Crude methanol extract of Solanum torvum was partitioned into petroleum ether (STF_1), chloroform (STF_2), ethyl acetate (STF_3), aqueous (STF_4) and STF_5 (_(precipitate)) fractions. STF_1, STF_2 and STF_3 were each subjected to vacuum liquid chromatography to obtain STF_(11- 17), STF_(21- 28) and STF_(31- 37). The crude extract STF_0, partition fractions STF_(1-5) and sub fractions STF_(11- 17), STF_(21- 28) STF_(31- 37) were assayed for anti-trichomonal activity. The crude extract, STF_0, at 24 h and 48 h showed LC_(50) and LC_(90) of 386.34±17.96, 2168.31±119.43, 85.81±20.10, 324.72±17.73 (μg/mL), respectively. STF_5 _((precipitate)) had LD_(50) and LD_(90) at 24 and 48 h as 25.84±5.12, 70.36±15.64 and 13.87±1.61, 42.94±6.20 (μg/mL), respectively. STF_1, STF_2 and STF_3 had LD90 at 48h as 72.76±1.72, 119.39±3.90 and 114.54±36.08 (μg/mL) while STF_(13), STF_(14), STF_(23) and STF_(34) had corresponding values of LD_(90) at 48h as 67.18±14.90, 88.07±4.78, 89.95±7.99 and 84.03±8.03 (μg/mL). The partition fractions STF_1, STF_2 and STF_3, the VLC sub fractions STF_(13), STF_(14), STF_(23), STF_(34) and the standard drug did not show significant difference (p＞0.05) in their bioactivities. It is concluded that methanol extracts of the fruits of Solanum torvum are a potential source of drug (s) for the treatment of trichomoniasis.