This study is to design and develop a semi-automatic sound insulation measuring system, and analyze this system's uncertainty of measurement using a uniform signal layer of material. We adapt the semi-automatic and manual placement measuring methods and analyze the repeatability and reproducibility of sound transmission loss data using single layer polycarbonate panels. We also take acoustical software simulation calculation and compare the experimental results using calcium silicate board. Finally, we adapt the semi-automatic measuring system and use scanning and fixed points testing for a series of windows type in the domestic market. The results shown that the measurement procedure with shorter time can be finished and the accuracy of measurement data can be confirmed for the semi-acoustic measuring system.