- 期刊
- OpenAccess
Inhibition Activities of Papaya Black Seed Extracts on Colorectal Cancer Cell Viability and Migration
摘要
Papaya (Carica papaya L.) is a rich-nutrition fruit including vitamins (A, B and C), carbohydrates, proteins, alkaloids, proteolytic enzymes, and benzyl isothiocyanate (BITC). BITC is a bioactive substance and it presents in the papaya seeds. It also has applied in many areas according to its bioactive abilities. The aim of this study is the investigation of the effect of papaya black seed oil on anti-colorectal cancer cell viability and migration. One CT-26 colorectal cancer cell line was used in this study. The black seeds from yellow (ripe) papaya were harvested and then extracted by the supercritical fluid CO_2 extraction. The analysis of extract content, including fatty acids, BITC, and other components, was performed using comprehensive two-dimensional gas chromatography coupled with time of flight mass spectrometry (GC-GC-TOFMS). Assay for cell viability and migration were MTT [3-(4, 5-Dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide] cell viability assay and wound-healing assay, respectively. The supercritical fluid CO_2 extracts from papaya black seeds significantly decreased cell viability and migration of CT-26 colorectal cancer cell line. The data suggest that the supercritical fluid CO_2 extracts of papaya black seeds have anti-cancer cell viability and migration effects on colorectal cancer cells. The anticancer effect of papaya black seeds may result from its high concentration of BITC (405.43 μg/g papaya black seed extracts). Taken together, papaya black seed extracts may have a potential to inhibit the growth of colorectal cancer cells.