- 期刊
市售蒼朮藥材之鑑別及其化學成分含量之測定
Identification of Aractylodes Rhizoma by Pharmacognosy and Determination of Atractylodin by UPLC
摘要
蒼朮(Aractylodes Rhizoma)為菊科茅蒼朮Aractylodes lancea (THUNB.)DC.或北蒼朮Aractylodes chinensis (DC.)Kcidz.的根莖。大陸產之菊科蒼朮屬(Aractylodes)植物有多種，其藥村在型態上非常相似，不易鑑別，而台灣市售藥材多由大陸進口，為了解市售蒼朮之基原及指標成份含量，本計畫價購全國北、中、南區藥廠及藥房蒼朮藥材共50件，參照文獻之記載，經性狀特徵、組織鏡檢及薄層層析法鑑別，50件檢體皆為正品北蒼朮藥材，再以極致效能液相層析法(UPLC)分析檢測藥材中蒼朮素(Atractylodin)之含量，最後開發10種蒼朮複方濃縮製劑中蒼朮素快速檢測定量法。 本實驗之極致效能液相層析法係採用Acquity BEH C18、2.1mm×100 mm，1.7μm管柱，流動相為乙腈－水溶液，利用線性梯度沖提，檢測波長為330 nm。線性迴歸方程式Ｙ=207.195X-0.247(r^2=0.9998)，呈良好線性關係，同日間(Intraday)相對標準偏差介於0.852~0.962%，異日間(Interday)相對標準偏差介於0.964~1.369%。市售蒼朮藥材50件，利用上述極致效能液相層析法分析蒼朮素含量介於0.064~0.388%間；蒼朮複方濃縮製劑，其線性迴歸方程式Y=77.289X+0.018(r^2=0.9994)，同日間相對標準偏差介於0.471~0.534%間，異日間相對標準偏差在0.708~1.562%間，其蒼朮素含量在0.024~0.229%間。
並列摘要
The botanical origin of Aractylodes Rhizoma is dried rhizome of Aractylodes lancea (THUNB.) DC. and Aractylodes chinensis (DC.) Kcidz. Because of the morphological similarities of Aractylodes species, alternatives and adulterants were found and reported in literatures. In order to identify the botanical origins of commercial Aractylodes Rhizoma in Taiwan, 50 samples, purchased from market, were examined and compared with authentic materials by morphology, microscopy and thin layer chromatographic analysis. Identification approaches were thus established in this study and applied to identify botanical origins of samples. The results showed that all of 50 samples (100 %) were Aractylodes Rhizoma. Atractylodin in crude drugs and Chinese medicinal preparations was examined by Ultra Performance Liquid Chromatography. Samples were analyzed on a 1.7 μm ACQUITY BEH C18 reversed phase column with a gradient elution using varied proportion of water and acetonitrile as mobile phase and monitored at 330 nm. Regression equations revealed the linear relationship with correlation coefficients of 0.9994 and 0.9998 between the peak-area ratios of atractylodin to internal standard and concentration of atractylodin in crude drugs and Chinese medicinal preparations, respectively. The relative standard deviations of atractylodin ranged between 0.852~0.962%, 0.471~0.534% (intraday) and 0.964~1.369%, 0.708~1.562% (interday), respectively. The contents of the atractylodin in 50 crude Aractylodes Rhizoma samples were 0.062~0.388%, while in 10 Chinese medicinal preparations of Aractylodes Rhizoma were 0.024~0.229%.