- 期刊
化粧品中合成及天然色素檢驗方法之建立
Establishment of an Analytical Method for Synthetic and Natural Colorants in Cosmetics
摘要
化粧品中色素主要用於改善產品觀感及提升消費者吸引力，合成色素因顏色多樣、著色力強及穩定性好被廣泛用於指甲油、眼影及唇膏等化粧品中。本研究係利用液相層析串聯質譜法（Liquid chromatography/tandem mass spectrometry，LC-MS/MS）建立一個簡單、快速之定性檢驗方法，可同時分析Acid orange 10等5項合成及天然色素成分。檢體以去離子水：二甲基亞碸（Dimethyl sulfoxide，DMSO）：甲醇（2：3：5，v/v/v）溶液萃取，以超音波振盪30分鐘，經濾膜過濾後，以LC-MS/MS進行分析。使用ACQUITY HSS T3層析管柱，並採用10 mM甲酸銨溶液及甲醇：乙腈（1：1，v/v）溶液作為移動相進行梯度沖提；利用電灑游離法（Electrospray ionization，ESI），搭配多重反應偵測模式（Multiple reaction monitoring，MRM）偵測，5項色素可在15分鐘內完成分析。以唇膏、唇蜜及指甲油等檢體作為化粧品研究基質，評估本方法偵測極限及專一性，結果5項色素於唇膏、唇蜜及指甲油之偵測極限皆為200 μg/g；且無基質干擾情形，添加標準品後分析5項色素成分之滯留時間及相對離子強度均能與標準品分析結果相符，顯示方法具有良好專一性。本檢驗方法可應用於上市後產品之監測，以確保消費者之健康安全。
並列摘要
Colorants are key components of cosmetics to enhance attractiveness to the consumers; synthetic dyes are extensively employed in makeup cosmetic products such as nail polish, eyeshadows, and lipsticks due to their diverse range of colors, strong coloring capabilities, and excellent stability. A simple and fast qualitative liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of acid orange 10, four other synthetic and natural colorants in cosmetics. The sample was uniformly dispersed with a solution of water: dimethyl sulfoxide: methanol (2:3:5, v/v/v) and then extracted by ultrasonication for 30 minutes. After filtration, the filtrate was separated on an ACQUITY HSS T3 column with gradient elution of 10 mM ammonium formate and methanol: acetonitrile (1:1, v/v) solutions. The analysis could be accomplished within 15 minutes by employing electrospray ionization (ESI) with multiple reaction monitoring (MRM) mode for detection. The detection limits and specificity of this method were assessed using a lipstick, a lip gloss, and nail polish as blank matrices. The results showed that the detection limits for the 5 colorants in lipstick, lip gloss, and nail polish were all at 200 μg/g. The blank samples showed no interference, and the retention times and relative ion intensities of the 10 colorants spiked in blank samples were consistent with those of 10 colorant standards, indicating that the method had good specificity. This method can be applied to post-market surveillance and safeguard health and safety of the consumers.