The Ganoderma Extracts from Tein-Shan Ganoderma Capsule Suppressed LPS-Induced Nitric Oxide Production in RAW264.7 Cells

天生靈芝萃取液可透過NF-kB路徑抑制細菌脂多醣誘發小鼠RAW264.7免疫細胞株的一氧化氮生成

朱建華(Chien-Hua Chu) ； 葉舒平(Shu-Ping Ye) ； 吳慧中(Hui-Chung Wu) ； 邱啟銘(Chi-Ming Chiu)

《MC-Transaction on Biotechnology》 7卷1期 (2015/03) Pp. 1-11

摘要

靈芝是一種東方傳統中藥主要功效為抗癌與免疫調節作用。本篇研究針對天生靈芝產品是否具有免疫調節功效進行探討；我們採用小鼠單核球／巨噬細胞株RAW264.7做為研究工具。研究發現天生靈芝粉末萃出液在建議用量範圍內不會影響免疫細胞存活率，但是在高濃度甲醇萃取液會造成免疫細胞型態變化。此外，靈芝水萃液與甲醇萃液均可有效抑制免疫細胞因細菌脂多醣誘發的一氧化氮釋出，這結果顯示此產品具有免疫抑制調節效果。報導基因研究發現此調控方式可能經由NF-kB路徑對一氧化氮生成酵素iNOS產生抑制作用。因此，此研究提供初步證據確定天生靈芝對免疫發炎反應具有抑制功效。

關鍵字

靈芝 ； 免疫調節 ； 發炎 ； 一氧化氮

並列摘要

Ganoderma lucidum is a high value mushroom used in oriented medicine for its anti-tumor and immunoregulatory effects. Here we determined one commercial Ganoderma product, Tein-Shan Ganoderma (TSG), for its immunomodulatory activity. In murine monocytic RAW264.7 cell line model, we found that the extracts of TSG did not affect cell viability of macrophages but caused cell morphology change under the treatment of TSG methanol extracts. Moreover, TSG extracts significantly inhibited nitric oxide (NO) synthesis in RAW264.7 cells stimulated by LPS. The suppression of NF-kB activation by LPS is correlated with the reduction of NO production under TSG administration. Our study shows that TSG plays a role in the suppression of inflammatory response in monocytic cells.

並列關鍵字

＂Ganoderma lucidum＂ ； immunomodulation ； inflammation ； nitric oxide ； NF-kB