番茄中不同Ty基因組合對於番茄黃化捲葉泰國病毒種之抗病力研究

Study on the Disease Resistant Ability to Tomato Yellow Leaf Curl Thailand Virus (TYLCTHV) in Tomato Lines with Different Combinations of Ty Genes

林煜恒(Yu-Heng Lin) ； 吳靜霞(Ching-Hsia Wu)

《臺中區農業改良場研究彙報》 139期 (2018/06) Pp. 13-26

摘要

選育對於番茄黃化捲葉泰國病毒種(Tomato yellow leaf curl Thailand virus, TYLCTHV)具抗病能力之番茄品種，為臺灣番茄抗病育種重要方向之一。瞭解番茄中不同Ty基因組合對於TYLCTHV之抗（耐）病能力，有助於番茄抗TYLCTHV育種工作。本研究中單獨帶有Ty1基因及部分單獨帶有Ty3基因的番茄品系於接種TYLCTHV後，植株發病率及病毒檢出率皆為0%；部分單獨帶有Ty2基因之番茄品系，於接種後第28天病徵達3.7級，且植株發病率及病毒檢出率皆為17%；單獨帶有ty5之番茄品系雖於接種後各時期皆無病徵表現，然植株TYLCTHV檢出率為80%；同時帶有Ty2及Ty3之番茄各品系於接種TYLCTHV後各階段無病徵表現，其病毒檢出率最低為30%；同時帶有Ty2及ty5基因之番茄品系，於接種後各階段無病徵表現，然病毒檢出率為91%；同時帶有ty5及Ty6基因之番茄品系，於接種後各階段無病徵表現，病毒檢出率最低為17%。由研究結果可推論，臺灣進行番茄抗TYLCTHV育種時，如同時堆疊Ty1、Ty2及Ty3基因或ty5及Ty6基因，可使番茄在感染TYLCTHV後有最低之植株發病率及病毒檢出率，而使育成之番茄新品種（系）對於TYLCTHV有最佳抗（耐）病能力。

關鍵字

番茄 ； 抗病育種 ； 番茄黃化捲葉泰國病毒種 ； Ty基因

並列摘要

Breeding tomato varieties (lines) resistant to tomato yellow leaf curl Thailand virus (TYLCTHV) is one of the important strategies for disease resistance breeding of tomato in Taiwan. Understanding the disease resistant ability to TYLCTHV among different combinations of Ty genes in tomato will be helpful for TYLCTHV resistance breeding. In this study, the disease incidence and virus detected rate of the tomato breeding line with single Ty1 gene and part breeding lines with single Ty3 were showed both 0% at 28 days after inoculating with TYLCTHV. Some of the tomato breeding lines with single Ty2 gene showed 3.7 of severity index on 28 days after inoculation with the plant disease incidence and virus detected rate both 17%. The breeding line with single ty5 gene showed no disease symptoms in each stage after inoculation, the detection rate of TYLCTHV in the plants was 80%. The tomato breeding lines with the Ty2 and Ty3 gene combination showed no disease symptoms in all stages after inoculation with TYLCTHV, and the lowest virus detection rate was 30%. The tomato line with the Ty2 and ty5 gene combination showed no disease symptoms in all stages after inoculation, but the virus detection rate was 91%. The tomato lines with the ty5 and Ty6 gene combination all showed no disease symptoms in all stages after inoculation, and the lowest virus detection rate was 17%. The results showed the best TYLCTHV resistance ability of tomato varieties (lines) can be obtained by pyramiding with Ty1, Ty2 and Ty3 genes, or pyramiding with ty5 and Ty6 genes in tomato TYLCTHV resistance breeding procedure in Taiwan.

並列關鍵字

tomato ； disease resistance breeding ； tomato yellow leaf curl Thailand virus (TYLCTHV) ； Ty gene