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Development of bio-pesticide Bacillus amyloliquefaciens ML15-4 to control of the graymold(Botrytis cinerea) of strawberry(Fragaria x ananassa)



Parallel abstracts

Strawberry (Fragaria x ananassa Duch.} is one of the most economically important fruit crops in the world. However, the necrotrophic air-borne fungus, Botrytis cinerea, which can affects many plant species, occurs abundantly throughout the year as a saprophyte and facultative parasite on strawberry. In this study, the Bacillus amyloliquefaciens ML15-4 isolated by the Miaoli District Agricultural Research and Extension Station was formulated as the bio-pesticides for control of Botrytis cinerea. Specifically, we optimized the five-tons of industrial-grade production processes and completed two bio-pesticide formulations of suspension concentrate and wettable powder. We proved that 1 X 10^9 CFU/ml of viable bacteria can be maintained at least for 24 months at room temperature (28°C ). The data of storage stability evaluation indicated that shelf life of the suspension concentrates and wettable powder was at least 2 years in duration at room temperature. In the greenhouse experiments, the disease severity of strawberry fruit gray mold (SFGM) in the early stage could be reduced about 12.8% by the ML15-4 suspension concentrates. To further evaluate the efficacy of the two bio-pesticide formulations on controlling SFGM, three preventive application experiments in fields were conducted. The results indicated that the applications of the formulated B. amyloliquefaciens ML15-4 (in 1:300 dilution) significantly reduced the disease severity of SFGM up to 54% to 58.7% in the field trials. The two bio-pesticide formulations of ML15-4 without residual toxin have the potentials to serve as the safety management methods for enhancing fruit quality and reducing the economic impacts of SFGM on the strawberry industry. We expect that the two bio-pesticide formulations of ML15-4 could provide an alternative approach to chemical pesticides in management of SFGM more safely and reliably.