The relationship of multiple sclerosis and chronic craniospinal venous insufficiency (CCSVI) is controversy. The purpose of this study was to report the prevalence of CCSVI in patients who received 3D contrast enhanced magnetic resonance venography in our databank, and to compare the prevalence between subjects with and without multiple sclerosis (MS). Retrospectively, a total of consecutive 27368 brain MR examinations were reviewed in our MR data bank. We defined CCSVI as those patients had venous reflux into the internal paraspinal venous plexus and also >80% stenosis of the internal jugular vein. The patients with CCSVI were divided into two groups (MS and non-MS). The levels of significant stenosis were classified as at C1-2, only at carotid bulb long segment between C1-2 to carotid bulb, and at distal internal jugular vein (IJV). After excluding those un-qualified studies according to our exclusion criteria, 7024 studies were included in this study. They included the 161 patients diagnosed as MS were examined in the same period. A total of 18 patients found to have CCSVI according to the criteria mentioned above. The prevalence was 0.26% (18/7024). Among them, eleven were diagnosed to have MS. The prevalence of CCSVI in MS group was 6.8% (11/161) in this particular group. The prevalence of CCSVI in the group without MS was 0.1% (7/6863). CCSVI occurred more in male patients (77.8%), and significant stenosis was found in a total of 24 IJVs and 88.9% were on the left side. A total of 36 sites of stenosis noted. In 8 of MS group and 4 of non-MS group, two stenosis in a single IJV was found. CCSVI does not occur solely in patients with MS. The prevalence of CCSVI in MS group is higher than in non-MS group.