PURPOSE. Double-chambered right ventricle (DCRV) is a disorder when hypertrophied muscle bundles divide the right ventricle into two chambers, causing a variable degree of obstruction. When treated properly with surgery, DCRV is a disease with great prognosis. This study is aimed to investigate if dynamic cardiac computed tomography (CCT) improves the diagnostic accuracy of DCRV as compared to transthoracic echocardiography and static CCT, and to establish a diagnosing criterion. MATERIALS AND METHODS. Retrospective measurement of subinfundibular right ventricular outflow tract (RVOT) diameters during end-systole and end-diastole on dynamic CCT of 60 children with ventricular septal defects (VSDs) was conducted in a single institution. Transthoracic echocardiography, static CCT and surgical records of the 60 patients were also reviewed and analyzed. RESULTS. One-third (n = 20) of the patients had associated DCRV according to their surgical findings. The most effective single factor to predict DCRV was when the height (mm)-corrected subinfundibular RVOT diameter (mm) during end-systole became less than 0.008 (sensitivity = 80.0%; specificity = 97.4%). All parameters measured during end-systole have a relatively better DCRV diagnostic value than those measured during end-diastole. Transthoracic echocardiography, static CCT and dynamic CCT showed respective diagnostic accuracies of 78.3, 80.0 and 91.7%. CONCLUSION. Dynamic CCT with end-systolic measurements proved to be the better imaging modality for the diagnosis of DCRV with higher accuracy, as compared to transthoracic echocardiography and static CCT.