- 期刊
Multiple Enzyme Doped Thread-based Microfluidic System for Blood Urea Nitrogen and Glucose Detection in Human Whole Blood
並列摘要
This research presents a novel technique for detection human whole blood utilize a novel enzyme-doped thread with PVC (Polyvinylchloride) membrane coating for on-site urea and glucose detection on a thread-based microfluidic device. The enzyme can be directly applied on the thread without delicate pretreatment or surface modification process. The passing biomolecules are digested by the enzymes and then electrochemically detected downstream. With this approach, CE-EC detection with on-site bio-reaction can be simply achieved. Whole blood sample is firstly mixed with RBC lysis buffer to prevent from blood coagulation. The lysed RBC and other solid pieces is simultaneously filtered away while electrokinetically flowing in the thread-based microfluidic system. A thin layer of PVC membrane is coated on the enzyme-doped thread to further fix the applied enzyme and to prevent from the rapid evaporation of the running buffer due to the Joule heating effect. In addition, the PVC coated thread can be operated at a higher separation electric field of 500 V/cm due to the reducing buffer evaporation.