In this work, multiple regression analysis was carried out via experiments and data analysis to attain the optimal reacting conditions of the KMnO_4-H_2SO_4-Na_2SO_3 chemiluminescence system for acrylamide concentration detection through static injection analysis, i.e. KMnO_4:3.8×10^(-4)mol/L; Na_2SO_3:1.8×10^(-3)mol/L; H_2SO_4:3.6×10^(-2)mol/L. In addition, the correlation and the linear regression equation of acrylamide concentration and relative luminous intensity were obtained under the optimal reacting conditions.