- 期刊
檢測牡蠣中腸炎弧菌的帶菌率及藥敏型式
The Carrier Rate and Antibiograms of Vibrio parahaemolyticus Isolated from Oysters Sold in Taipei Area Markets
摘要
腸炎弧菌（Vibrio parahaemolyticus）為食品中常見之致病性細菌，若生食或食用未完全煮熟之海鮮類產品，且該產品受此菌汙染，便可能造成食物中毒；本研究係針對新北市林口區、泰山區及新莊區市場所販售之牡蠣共30件檢體進行腸炎弧菌檢測。依據衛生署公告的食品微生物之檢驗法－腸炎弧菌之檢驗，檢測過程包括增菌、移種、分離、生化試驗及Multiplex PCR之一連串步驟，檢測結果發現腸炎弧菌陽性之件數為9件（帶菌率為30%，9/30）。進一步針對檢體來源進行分析，發現自傳統市場之商家購得之20件牡蠣中，有7件檢出腸炎弧菌陽性（帶菌率為35%），而另外10件購自超級市場的牡蠣則有2件檢出腸炎弧菌（帶菌率為20%）。隨後對分離之9株腸炎弧菌以multiplex PCR方法對菌種進行確認以及對致病基因包括耐熱性溶血素（thermostable direct hemolysin, tdh）及類耐熱性溶血素（thermostable related hemolysin, trh）進行鑑別，結果並未發現任何一株菌具有致病基因。進一步將9株腸炎弧菌進行瓊脂紙錠擴散試驗之藥敏試驗，顯示腸炎弧菌對penicillin G、ampicillin及cephalothin具有抗藥性，而對tetracycline、chloramphenical、gentamicin、kanamycin及nalidixicacid具敏感性。雖然本研究發現從牡蠣分離的腸炎弧菌致病危險並不高，但由於台灣爆發由海鮮媒介的腸炎弧菌食物中毒經常發生，民眾仍然需要注意避免食用未煮熟的海鮮產品。
並列摘要
Vibrio parahaemolyticus is the most frequently encountered pathogen that can cause gastroenteritis in humans. It is often found in bivalve mollusks such as oysters. Consuming raw or poorly cooked seafood contaminated with this microorganism can cause food poisoning. The aim of this study was to investigate the carrier rate of V. parahaemolyticus in oysters sold in Lin-Kou, Tai-Shan, and Xin-Zhuang districts in New Taipei City. Thirty samples of oysters were examined in the present study. We followed TFDA guidelines on ＂Food Microbial Testing Methods-V. parahaemolyticus examination＂ to enrich, transfer, isolate, and purify the bacterium, and then to perform biochemical tests and multiplex polymerase chain reaction (PCR). We found nine positive specimens, representing a carrier rate of 30% (9/30). We further analyzed the sources of this organism and found that there were 7 positive specimens among 20 oysters (35%, 7/20) purchased from traditional markets and 2 among 10 oysters from supermarkets (20%, 2/10). Afterward, we confirmed their identities, conducting tests to analyze the pathogenic genes, thermostable direct hemolysin (tdh), and thermostable related hemolysin (trh) for all nine isolated strains using multiplex PCR methods. The results showed that none of the strains carried the pathogenic genes. We then performed the antibiograms for all of those nine isolated strains. The results indicate that V. parahaemolyticus is resistant to penicillin G, ampicillin, and cephalothin but susceptible to tetracycline, chloramphenicol, gentamicin, kanamycin and nalidixic acid.