- 期刊
以複製接種器操作CAMP試驗應用於高通量Streptococcus agalactiae, Listeria monocytogenes及Clostridium perfringens之鑑定
High-throughput Identification of Streptococcus agalactiae, Listeria monocytogenes and Clostridium perfringens by Replicator CAMP Test
摘要
CAMP（Christie, Atkins, Munch-Peterson）試驗用於偵測一種擴散性胞外溶血性之穩定性蛋白質（CAMP因子）。它可作為Streptococcus agalactiae（無乳鏈球菌，B群鏈球菌）與Listeria monocytogenes（單核球增多性李斯特菌）的初步鑑定依據，而逆向CAMP試驗（reverse CAMP test）則利用S. agalactiae所產生的CAMP因子偵測Clostridium perfringens（產氣莢膜梭菌）的α-溶血素（hemolysin）。由於臨床或食品檢體的分離培養基可能含有多個上述微生物生長菌落，因此，可同時操作數個菌落的高通量CAMP或逆向CAMP試驗將可節省檢驗耗材及人力。在本研究中，吾等以複製接種器（replicator）分別操作21株S. agalactiae,21株L. monocytogenes多個菌落之CAMP試驗，並操作1株C. perfringens之多重複菌落的reverse CAMP試驗，結果指出S. agalactiae, L. monocytogenes與C. perfringens試驗陽性率分別與100%, 66.7%, 100%。因此，以複製接種器操作CAMP試驗將適合S. agalactiae和C. perfringens的大量篩檢，而在L. monocytogenes的偵測應用需加以改進。
並列摘要
The CAMP (Christie, Atkins, Munch- Peterson) test is used to detect a thermostable, extracellular, and diffusible protein (CAMP factor) and can be used for initial identification of Streptococcus agalactiae (Group B streptococci, GBS) and Listeria monocytogenes. The reverse CAMP test uses the CAMP factor produced by S. agalactiae to detect the α-hemolysin of Clostridium perfringens. Since the isolation medium for clinical or food samples may contain the aforementioned microorganisms, a high-throughput CAMP or reverse CAMP test that can examine several colonies simultaneously would save laboratory consumables and labor. In this study, we used a replicator to conduct the CAMP test on multiple colonies of 21 S. agalactiae and 21 L. monocytogenes isolates and reverse CAMP test on multiple colonies of 1 C. perfringens isolate. Results showed that the positive rates for the identification of S. agalactiae, L. monocytogenes, and C. perfringens were 100%, 66.7%, and 100%, respectively. Therefore, the replicator CAMP test is suitable for mass screening of S. agalactiae and C. perfringens, while its application on the detection of L. monocytogenes needs to be improved.