- 期刊
Tween 80對常見乳酸菌之生長與活性的影響
The Effects of Tween 80 on Bacterial Growth and Viability of Common Lactic Acid Bacteria
摘要
Tween 80是微生物培養時常見的添加物，用以提高微生物的生長，並為食品與化妝品常用之乳化劑或藥品賦形劑。常見的乳酸菌菌株Lactobacillus acidophilus、Lacticaseibacillus paracasei和Lactiplantibacillus plantarum需在含有Tween 80的培養液中才能生長。為了探討此三菌種與臨界膠束濃度（critical micelle concentration, CMC）之關聯性，本研究測試不同濃度的Tween 80（0.05%、0.1%、0.5%和1%）對此三菌株生長的影響，結果指出L. acidophilus 與L. plantarum在含有0.1% Tween 80濃度之Tryptone-Yeast-Glucose（TYG）培養液的厭氧環境展現較高活的細胞數，而L. paracasei則在0.5%具有較高的活細胞數。利用平板計數法測定不同菌種在不同濃度Tween 80的世代期，結果發現L. paracasei在0.5%與1% Tween 80濃度的培養液中生長較為緩慢，而L. acidophilus與L. plantarum則無明顯差異。總之，測試結果指出添加不同濃度Tween 80的TYG培養液對於乳酸菌的厭氧生長呈現種的差異性，而與CMC之間無明顯關聯性。
並列摘要
Tween 80 is a common additive in microbiological cultures which used to enhance the growth of microorganisms. It is also widely used as an emulsifier in food and cosmetic products or pharmaceutical excipient. Common lactic acid bacteria, including Lactobacillus acidophilus, Lacticaseibacillus paracasei and Lactiplantibacillus plantarum require Tween 80 in their culture medium. This study aimed to investigate the relationship between these strains and the critical micelle concentration (CMC) by examining the effects of different Tween 80 concentrations (0.05%, 0.1%, 0.5%, and 1%) on their growth. The results indicate that in an anaerobic environment with Tryptone-Yeast- Glucose (TYG) culture medium containing 0.1% Tween 80, L. acidophilus and L. plantarum exhibit higher viable cell numbers, while L. paracasei shows higher viable cell numbers at 0.5% Tween 80 concentration. The results showed that there was no significant difference in generation time between L. acidophilus and L. plantarum when assessed using the plate counting method. However, there was better cell viability observed in TYG medium containing 0.1% Tween 80. On the other hand, L. paracasei exhibited better cell viability in the culture medium containing 0.5% Tween 80. The culture medium with different concentrations of Tween 80 showed species-specific effects in the growth and cell viability of the examined lactic acid bacteria.