- 期刊
用非黏蛋白（mucin）培養基量產次世代益生菌Akkermansia muciniphila
Mass Production of Next-Generation Probiotic Akkermansia muciniphila with a Non-mucin Culture Medium
摘要
Akkermansia muciniphila（Akk）為次世代益生菌（next-generation probiotics）之一，已被證明具有預防和改善肥胖、糖尿病、代謝症候群和發炎等健康問題的潛力。由於AKK是一種絕對厭氧的革蘭氏陰性菌，其量產非常具有挑戰性。原生AKK利用腸道中的黏蛋白（mucin）進行生長，因此傳統上都是在含有黏蛋白的培養基中進行培養。本研究比較從健康人體內分離出的一株AKK菌株（isolated strain）與參考菌株（BAA-835）在50升發酵槽中的生長情況，發酵槽中使用的是不含黏蛋白的磷酸鹽緩衝培養基（phosphate-buffered culture medium），其中含有葡萄糖、酵母萃取物、N-乙醯葡萄糖胺（N-acetyl glucosamine）和L-蘇胺酸（L-threonine）作為碳源和蛋白質源。經過42小時的培養，Isolated strain發酵液中的菌數（4.1×10^9 CFU/mL）高於BAA-835（2.3×10^9 CFU/mL）。然而，離心後，BAA-835菌泥中的菌數（9.1×10^(10) CFU/mL）高於分離株的菌數（1.7×10^(10) CFU/mL）。與保護劑（protectants）混合後，Isolated strain的凍乾菌數（5.9×10^(10) CFU/g）高於BAA-835的凍乾菌數（2.2×10^(10) CFU/g）。在30℃下保存一個月後，凍乾粉中BAA-835的菌數減少73%（降至6.0×10^9 CFU/g），而isolated strain的菌數減少了94%（降至3.3×10^9 CFU/g）。這是利用非黏蛋白培養基量產AKK的首篇報導。
並列摘要
Akkermansia muciniphila (Akk) is one of next-generation probiotics and has been shown to have the potential to prevent and ameliorate health problems such as obesity, diabetes, metabolic syndrome, and inflammation. As AKK is a strict anaerobe Gram-negative bacterium, its mass production has been very challenging. Since native AKK uses mucin in the intestine for growth, it is traditionally cultured in medium with mucin. In this study, we compared the growth of an AKK strain, which we had isolated from a healthy person, to that of the reference strain (BAA-835) in a 50-L fermenter with a non-mucin, phosphate-buffered culture medium containing glucose, yeast extract, N-acetyl glucosamine, and L-cysteine as carbon and protein sources. After 42 hours of cultivation, the bacterial count in the fermentation broth of the isolated strain (4.1×10^9 CFU/mL) was higher than that of BAA-835 (2.3× 10^9 CFU/mL). However, the bacterial count in the pellet of BAA-835 (9.1×10^(10) CFU/mL) was higher than that of the isolated strain (1.7×10^(10) CFU/mL). After mixing with protectants, the freeze-dried bacterial count of the isolated strain (5.9×10^(10) CFU/g) was higher than that of BAA-835 (2.2×10^(10) CFU/g). After one month of storage at 30℃ , the bacterial count of BAA-835 in the freeze-dried powder was decreased by 73% (to 6.0×10^9 CFU/g), while that of the isolated strain was decreased by 94% (to 3.3×10^9 CFU/g). This is the first reports of AKK mass production with a non-mucin culture medium.