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蛋白質體之電泳技術

Electrophoretic Techniques for Proteomics

摘要


於本文中,我們簡單回顧二維凝膠電泳與毛細管電泳分析蛋白質體之技術。二維凝膠電泳雖具同時解析上千個蛋白質之能力,但受限於操作繁瑣、分離耗時、靈敏度差且不易自動化等缺點。而毛細管電泳與雷射誘導螢光技術或質譜技術之結合則具有快速、高靈敏度、易自動化等優點,但其解析能力則較不盡理想。因此我們深信結合直接電泳溶離、毛細管電泳、二維凝膠電泳、雷射誘導螢光和質譜之技術將有助於蛋白質體學之研究。

並列摘要


This paper briefly reviews two-dimensional gel electrophoresis ( 2-DGE) and capillary electrophoresis (CE) for proteomics. 2-DGE provides capability of resolving thousands of proteins in a gel, while it is tedious, time-consuming, in sensitive, and not easy for automation. On the other hand, CE in conjunction with laser-induced fluorescence ( LIF ) or with mass spectrometry ( MS) is advantageous over 2-DGE, including rapidity, sensitivity, and ease for automation, but provides relatively low resolving power. It is thus our belief that techniques combined with electroelution, CE, 2-DGE, LIF, and MS will be suitable for proteomics.

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