應用雙套式聚合酶鏈反應快速鑑定傷寒沙門桿菌

Rapid Identification of Salmonella typhi by Duplex Polymerase Chain Reaction

林建谷(Chien-Ku Lin)

《弘光學報》 32期 (1998/10) Pp. 81-96

https://doi.org/10.6615/HAR.199810.32.06

摘要

傳統以生化型及血清型試驗鑑定傷寒沙門桿菌之過程相當煩雜且費時，因此發展傷寒沙門桿菌之快速鑑定方法，在醫療及疫情防治工作上均有其必要性。本研究針對傷寒沙門桿菌是沙門桿菌屬中唯一同時具有Hl-d鞭毛基因及Vi毒力基因之血清型特性，發展特異性同時擴增此二基因之雙套式聚合鏈反應(PCR)。所測試之傷寒沙門桿菌以Hl-d基因 特異性引子組(HD3/HD4)及Vi基因特異性引子組(PIIF/PIlR)進行雙套式PCR，均可產生產物大小分別為280 bp及206 bp之產物:而其他僅具Hl-d基因(S. muenchen, S. florida等)或Vi基因(S. paratyphi C, S. dublin等)之菌株，則只產生個別PCR產物，此結果顯示此雙套式PCR系統 在快速鑑定傷寒沙門桿菌之特異性甚佳。

關鍵字

雙套式PCR ； 傷寒沙門桿菌 ； Hl-d鞭毛抗原 ； Vi抗原

並列摘要

The conventional biochemical and serological method for identification of Salmonella typhi is tedious and time-consuming. It is essential for medical remedy and epidemic control of typhoid infection by rapid and specific detection of S. typhi. Among Salmonella serotypes, S. typhi is the only one with both of Hl-d and Vi antigens. Therefore, this study was to develop a duplex PCR system for specific identification of S. typhi through simultaneous amplification of genes encoding Hl-d and Vi antigens. The PCR products based on amplification of Hl-d and Vi genes by HD3/HD4 plus PIIF/PIIR primer set were 280 bp and 206 bp, respectively. While, other strains with Hl-d or Vi gene could only generated corresponding PCR product. These results showed such duplex PCR system was specific for the identification of S. typhi.

並列關鍵字

duplex PCR ； Salmonella typhi ； Hl-d flagellin ； Vi antigen

參考文獻

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