- 期刊
以毛細管電泳測定蜂膠中類黃酮物質－分析條件之探討
Analysis of Propolis Flavonoids by Capillary Electrophoresis－Study of the Parameters
摘要
本文針對蜂膠中的類黃酮物質，探討毛細管電泳分析時最適條件。首先選擇apigenin、chrysin luteolin、galangin、kaempferol、morin、myricetin、quercetin、quercitrin、rutin、hesperetin、naringin、naringenin、daidzein及genistein等15種類黃酮商品作為標準品，以毛細管電泳進行分析，探討的條件包括偵測波長、緩衝液種類、緩衝液pH值、緩衝液濃度以及電泳分析模式。結果發現搭配毛細管區帶電泳(capillary zone electrophoresis, CZE)及膠束動力學毛細管層析(micellar electrokinetic capillary chromatography, MECC)兩種分離模式，可完全分離此15種類黃酮標準品。最適當的緩衝容液，在CZE為0.1 M H_3BO_3 (pH 9.5)-5% 甲醇，在MECC則為0.03 M Na_2B_4O_7-0.05 M SDS (pH 8.5)；最適溫度為25 ℃，最適偵測波長為214nm，使用電壓分別為18 KV (CZE) -15 KV (MECC)。分離效果之決定係數(R^2)達0.99以上。
並列摘要
This research was conducted to investigate the best conditions for analysis of propolis flavonoids by capillary electrophoresis. At first, 15 commercially available flavonoid standards including apigenin, chrysin, luteolin, galangin, kaempferol, morin, myricetin, quercetin, quercitrin, rutin, hesperetin, naringin, naringenin, daidzein and genistein were selected to develop the best electrophoretic conditions. The parameters studied included detection wavelength, buffer species, pH value of buffer solution, concentration of buffer solution and mode of electrophoresis. Results showed these flavonoid standards were completely separated by capillary zone electrophoresis (CZE) in combination with micellar electrokinetic capillary chromatography (MECC). The most appropriate buffer solution was 0.1 M H_3BO_3 (pH 9.5)-5% methanol in CZE, while 0.03 M Na_2B_4O_7-0.05 M SDS (pH 8.5) in MECC. The best temperature and detection wavelength were 25 and 214 nm, respectively. CZE was conducted at 18 KV and MECC at -15 KV. Coefficients of determination (R^2) in CZE were all above 0.99.