不同濃度之Calcium ionophore (A23187)對體外成熟豬卵母細胞激活與胚發育之影響

Effect of Calcium Ionophore (A23187) Concentration on the Activation and Developmental Maturation of Porcine Oocyte In Vitro

林育安(Yu-An. Lin) ； 廖明輝(Ming-Huei Liao) ； 陳銘正(Ming-Cheng Chen)

《弘光學報》 56期 (2009/08) Pp. 85-97

https://doi.org/10.6615/HAR.200908.56.08

摘要

本試驗之目的，乃研究不同濃度之calcium ionophore (A23187)對體外成熟豬卵母細胞之激活與發育之影響。豬卵母細胞經體外成熟培養48小時後，分別以0、100、200與300 μM之calciumionophore (A23187)激活5分鐘，隨後培養於NCSU-23胚培養液中16小時或144小時，檢查卵母細胞之激活與胚發育情形。試驗結果顯示，0、100、200 與 300 μM組卵母細胞之激活率分別為7.7、56.2、53.7與32.6% (P＜0.05)，100 μM組與200 μM組，彼此間無顯著差異，但當calcium ionophore (A23187)之濃度增加到300 μM時，卵母細胞之激活率則顯著下降(P＜0.05)。卵裂率與囊胚率方面，均以100μM組最高(P＜0.05)，300 μM組發育到桑椹胚階段就終止發育。

關鍵字

豬卵母細胞 ； 體外成熟 ； 孤雌激活

並列摘要

The objective of this study was to investigate the effect of calcium ionophore (A23187) concentration on the activation and developmental maturation of porcine oocyte in vitro. Porcines oocytes were used in an activation test with 0, 100, 200 and 300 μM calcium ionophore (A23187) for 5 min after maturation culture for 48 hours. Then, the oocytes were transferred into NCSU-23 embryo medium for another 16 hours or 144 hours incubation and then fixed and examined. The results indicate that the activation rates of 0, 100, 200 and 300 μM groups are 7.7%, 56.2%, 53.7% and 32.6% (P＜0.05), respectively. There was no significant difference among the activation rates of 100 μM group and 200 μM group. The activation rate was significantly decreased (P＜0.05), when calcium ionophore (A23187) concentration increased to 300 μM. In oocyte cleavage rate and blastocyst rate, 100 μM group was the highest among four groups (P＜0.05). The embryo of 300 μM group development to morula stage was stopped.

並列關鍵字

porcine oocyte ； in vitro maturation ； parthenogenetic activation

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