- 期刊
- OpenAccess
浸酸處理對不同胚胎期蠶卵孵化率之影響
Effect of Acid Treatment on the Hatchability of Bombyx mori Eggs at Different Embryonic Stages
摘要
家蠶(Bombyx mori L.) 品系C-18之休眠卵產後，在25℃溫度中施行5、30及60日之人工越夏後移至5℃冷藏越冬，越冬期間每隔7-8天，取出調查其胚胎形態並施以鹽酸浸漬處理後調查其25℃中催青之孵化率。試驗結果，冷藏初期，休眠中之蠶卵，施以浸酸處理後，未見促進孵化效果。休眠後期或越冬I期之卵，浸酸則可促進孵化；且浸酸時間較長者，效果較佳，但僅由胚胎形態，不能判別其休眠卵活化程度。越冬II、III期之胚胎，浸酸可促使蠶卵孵化整齊，浸酸時間長者，實用孵化率亦較高。越冬III期與臨界I期胚胎混合存在之卵，浸酸亦可使孵化整齊，惟浸漬3-7分鐘之實用孵化率無顥著差異。臨界I期及臨界II期之初，浸酸與否對實用孵化率無顯著影響。臨界II期之後期，當胚体已充分伸長，浸酸對卵之孵化即產生反效果，且浸酸時間越長者，孵化率越差。
並列摘要
Diapause eggs of the silkworm, Bombyx mori L., after being laid were kept at 25°C for 5, 30 or 60 days and then transferred to 5°C for overwintering. During the chilling period , the embryogenesis was investigated at 7- 8 day intervals, as was the hatchability with or without dipping in 20% HCI at 48 °C for 0-7 mim, followed by incubation at 25 °C. At the diapausing embryonic stage, acid treatment did not increase hatchability. In addition, hatchability increased with longer dipping time. At critical and primary critical II embryonic stages, acid treatment did not affect hatch ability, but it lowered hatchability after the terminal critical II stage. Furthermore, hatchability decreased with longer chilling days or HCI-dipping time.