- 期刊
- OpenAccess
影像分析探討餵食葡萄糖液對蟑螂心輸出量的效應
Effects of Glucose on the Cardiac Output of Cockroaches by Video Image Analysis
摘要
本研究的目的是利用影像擷取再輸入電腦，直接觀察昆蟲活體心臟的活動情形，並探討餵食葡萄糖液對美洲蟑螂心輸出量的效應。觀察時，先以影像分析測量實驗昆蟲心縮容積與心舒容積的變化，再換算心搏量（stroke volume）及心跳率（heart rate），最後算出心輸出量（cardiac output），並以不同濃度的葡萄糖液餵食雄性的成蟲為實驗組，以餵食蒸餾水或餵食葡萄糖液前的數據為對照組，觀察並比較心臟活動受餵食葡萄糖液的影響。結果發現濃度在5%以上的葡萄糖溶液，確實可引起心跳率顯著性上升，但心搏量沒有顯著性的差異。以10%葡萄糖溶液餵食（n = 10），蟑螂的心跳率增加14.37 ± 4.70％，具顯著差異（p ＜ 0.01），10分鐘後則漸趨回復到正常範圍；心搏量則增加1.67 ± 1.71%（p ＞ 0.05）；心輸出量增加14.13 ± 3.18%，也具有顯著差異（p ＜ 0.01）。餵食蟑螂葡萄糖溶液增加心跳率和心輸出量的機制，將在文中討論。
並列摘要
The cardiac activities of cockroaches (Periplaneta americana) were studied by video image analysis, and the effects of feeding a glucose solution on the cardiac output were investigated. Stroke volumes of insects were determined from differences in end-systolic and end-diastolic volumes of the heart; this was multiplied by heart rate to yield cardiac output. We investigated the effects of feeding glucose on the cardiac output of male adult insects. Control groups were fed distilled water, and experimental groups were fed glucose in various concentrations. The results show that the feeding of glucose solutions up to 5% in concentration significantly increased heart rate, but non-significantly affected stroke volume. Feeding a 10% glucose solution increased the heart rate by 14.37±4.70% compared with the feeding of distilled water (p ＜ 0.01, n = 10), but non-significantly increased stroke volume. Feeding a 10% glucose solution also increased cardiac output by about 14.13±3.18% compared to the control group (p ＜ 0.01, n = 10). The mechanism by which feeding glucose increases the heart rate and cardiac output of cockroaches is discussed.