In order to evaluate the hazards of radon to basement personnel more effectively and accurately, this paper takes a basement of a school laboratory as the research site, and completed the continuous measurement of radon concentration in different positions of the basement under different conditions, and the numerical simulation of indoor radon concentration under different ventilation rates was carried out based on the experimental conditions of basement model established by FLUENT software. As well as by comparing the simulation results with the experimental measurements, the influence of the opening and closing state of the basement door and the size of the ventilation rate on the distribution of indoor radon concentration is discussed and analyzed. The results show that the radon concentration in the basement is relatively high under the closed static condition, and the radon concentration is approximately uniform; the indoor radon concentration decreased significantly after the door is opened, but it is still higher than the outdoor level, and the indoor radon concentration continues to decrease and tends to the outdoor level after stabilization after ventilation.