Preparation and Histological Evaluation of Decellularized Adipose Tissue using Efficient Defatting and Decellularization Methods

Cheng Lu

《International Core Journal of Engineering》 6卷8期 (2020/08) Pp. 269-274

https://doi.org/10.6919/ICJE.202008_6(8).0036

摘要

Improve the efficiency of defatting and decellularization in the decellularization protocol to obtain a decellularized adipose tissue matrix with complete morphological structure. Evaluate the histological composition of decellularized adipose tissue and explore its possibility as a scaffold material for tissue engineering. A modified decellularize protocol was used to prepare decellularized adipose tissue from subcutaneous adipose of bovine. Oil red O staining, hoechst staining and H&E staining were performed on the decellularized adipose tissue. Quantitative detection of DNA, collagen and glycosaminoglycan content in decellularized tissues. And decellularized tissue remaining DNA, collagen and glycosaminoglycan content of quantitative detection. The decellularization efficiency of decellularized adipose was 97.3%, and it had a complete collagen structure. The decellularized adipose prepared by the modified method had a higher degree of defatting and decellularization, no cytotoxicity, and the natural structure and ingredients are kept intact.

關鍵字

Adipose tissue ； Decellularized ； Phospholipase A2 ； Tissue engineering

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