How does Matrine Affect the Survival Rate of Tumor Cell Lymphocytic Leukemia Tumor Cell and its Pathway

Honghan Xu

《International Core Journal of Engineering》 6卷12期 (2020/12) Pp. 297-301

https://doi.org/10.6919/ICJE.202012_6(12).0044

摘要

In recent years, traditional Chinese medicine has shown an important and indispensable role in the treatment of cancer due to its small side effects and low prices. Matrine, a chemical component extracted from plants in the legume plant, has been proved to have potential therapeutic value on lymphocytic cancer. The purpose of this experiment is to study the effect of matrine on promoting tumor cells apoptosis by using Jurkat cells and MOLT-4 cells and to detect the pathways of matrine in nude mouse's Jurkat cell tumor. Thus, it can provide theoretical basis for future study and clinical treatment. In this experiment, the Annexin V-FITC / PI staining was used to detect the apoptosis ratio of tumor cells and Western blot was used to detect the pathway of matrine molecules in nude mouse's tumor cell. This proposal includes 6 possible research results, 4 of them are the possible result in survival rate of tumor cells in both in-vivo study and in-vitro study: 1) Matrine induces both cancer cells apoptosis and has the optimal concentration; 2) Matrine induces only in-vitro cancer cells apoptosis and has the optimal concentration; 3) Matrine induces only in-vivo tumor cells apoptosis and has the optimal concentration; 4) Matrine successfully induces tumor cell apoptosis, but without accurate optimal concentration. The other two results are about study in pathway: 5) p38 MAPK is activated in the matrine-induced apoptosis pathway; 6) p38 MAPK is not activated in the matrine-induced apoptosis pathway.

關鍵字

Matrine ； Lymphocytic leukemia tumor ； Apoptosis ； Pathway

參考文獻

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