With the improvement of automatic container terminal, the application of straddle carrier has been more extensive. Compared with the AGV, the straddle carrier can independently complete the operations of pick-up, handling, stacking, loading and unloading, etc., which greatly improves efficiency of terminal. In this paper, the straddle carrier is used as the horizontal transportation equipment, and the operation way is loading and unloading mode. The mathematical model with the shortest total operation time of straddle truck operation was established. Tabu search algorithm and simulate anneal algorithm were used to solve the problem. Compared with the AGV operating system, the results show that, under loading and unloading mode, the straddle carrider operating system is more efficient with the same wharf configuration.