In order to study the jacking construction process of steel truss bridge across the existing expressway, this paper takes a simply supported steel truss railway bridge as the research background, and uses the finite element software to simulate the jacking construction process. By analyzing the mechanical performance, deformation and anti overturning stability of the steel truss bridge during launching, the safety and stability of the temporary support and its foundation are analyzed. The results show that the stress values of steel truss and steel guide beam are within the limits under each pushing condition; The deformation of each position of the guide beam and the safety and stability of the temporary support foundation meet the requirements of the construction. It provides reference for similar projects.