National defense education in Colleges and universities is an important way for college students' military theory education and practice. Integrating national defense education into campus culture cultivation system is a necessary means to adapt to the reform and improvement of educational methods in the new era. Through the research on the current situation of national defense education and campus culture in Colleges and universities, this paper probes into the existing problems of national defense education in Colleges and universities, and puts forward an effective way to integrate national defense education and campus culture.