Skin conductance response could be used as a clue to emotional formation. The measurement of skin conductance response enables us to objectively capture and observe the changes in emotional states based on physiological activation. Our research aims to create a new system of fear formation and elimination for epilepsy patients. In this study, participants experienced a two‐day conditioning and elimination paradigm of differential fear. One stimulus was fear conditioned reflex (CS+) while the other stimulus is not CS‐ in the acquisition stage. During the following extinction, both stimulus (CS+and CS‐) were presented to participants in the absence of the US. The next day, both kinds of stimulus appeared, and selective catalytic reduction was recorded during the recall test. We found that the subjects initially acquired fear of CS+, and then spread to fear of CS‐. Finally, after the extinction period, SCR decreased significantly, which makes the model system of fear formation and extinction technology for epileptic patients feasible.