This study examines whether the training investment has a positive effect on enterprise performance, taking enterprise life cycle as a moderator variable. The data covers the period of 2017-2019 for 204 publicly A-share listed high-tech firms in China. The findings indicate that enterprise life cycle has a significant moderating effect on the relationship between training investment and enterprise performance. In addition, in the growth stage, the more training budgets firms invest in, the better their performance will be. In contrast, the promotion effect of enterprise training investment on enterprise performance is weak in the decline stage and weakest in the mature stage. Therefore, the study shows that high-tech enterprises are supposed to adjust the training investment based on the accurate judgment of the life cycle.