Based on the Wenzhou science and technology innovation policies from 2000 to 2021, a three-dimensional model of release time-innovation subject-policy instrument was constructed and analyzed after quantifying the textual content of policy issuing institutions, release time, text form, policy instrument content, and innovation subject. The study found that: from the perspective of time, the number of science and technology innovation policies has generally been on the rise since 2000; from the perspective of innovation subjects; enterprises are the key target of policy benefits at each stage, while innovation platforms and parks are the key support objects of Wenzhou's science and technology innovation policies in the past five years; from the perspective of policy tools, environmental optimization and supply-demand tools account for the majority, while demand-satisfaction tools are under-applied. To improve the efficiency and effectiveness of the implementation of science and technology innovation policies, it is suggested that the legislative planning of science and technology innovation policies should be strengthened, the legislative level of science and technology innovation policies should be raised, and the layout of science and technology policies should be optimized from multiple angles and in all aspects, the use of demand-satisfaction tools should be increased and the introduction and training of science and technology talents should be emphasized.