台灣乳牛淋巴球黏力缺失症基因頻率

Gene Frequencies of Bovine Leukocyte Adhesion Deficiency of Holsteins in Taiwan

黃鈺嘉(Yu-Chia Huang) ； 張秀鑾(Hsiu-Luan Chang) ； 林德育(Der-Yuh Lin) ； 廖仁寶(Ren-Bao Liaw) ； 陳若菁(Jo-Ching Chen) ； 吳松鎮(Song-Cheng Wu) ； 楊德威(Te-Wei Yang) ； 黃金山(Chin-Shan Huang) ； 曾青雲(Chin-Yun Tseng) ； 蕭宗法(Thung-Fa Shiao) ； 劉秀洲(Hsiu-Chou Liu) ； 劉振發(Jenn-Fa Liou) ； 吳明哲(Ming-Che Wu)

《畜產研究》 33卷1期 (2000/03) Pp. 37-47

摘要

乳牛遺傳缺陷CD-18突變基因是引起牛淋巴球黏力缺失症（Bovine Leukocyte Adhesion Deficiency, BLAD）的隱性基因。CD-18突變點是第383個核苷酸由A（腺嘌呤）突變為G（鳥糞嘌呤），致遺傳轉譯時胺基酸由甘胺酸取代天門冬酸。台灣的生產用乳牛品種為荷蘭乳牛，自1997年7月起至1998年10月止共檢測了五個民間牧場的1387頭牛隻（北部兩場及南部三場），與北部與東部地區參與DHI計畫各牧場的71頭自留小公牛。牛隻的核內DNA萃取自全血血樣。全部1458頭牛隻中共測出98頭（6.7%）雜合型（BL）與1360頭正常型牛隻（TL）。71頭自留小公牛中共檢測出6頭雜合型小公牛（8.5%），而來自五個場的1387頭牛隻共測出92頭雜合型（6.6%）。這五個場的雜合型牛之頻率分別為2.9%（3/103），5.4%（19/350），5.5%（7/127），5.6%（5/89）與8.1%（58/718）。本研究調查樣本中未存在有病型（BLAD）個體，依本研究的數據可估計現階段台灣牛群中，淋巴球黏力缺失症的雜合性個體（BL）的頻率仍在5%以上。

關鍵字

乳牛 ； 牛淋巴球黏力缺失症 ； 聚合酶連鎖反應

並列摘要

Bovine Leukocyte Adhesion Deficiency (BLAD) is a genetic defect in dairy cattle. A point mutation (adenine to guanine) in position 383 is responsible for the defect, which results in a replacement of amino acid from aspartic acid to glycine. In Taiwan, Holstein is the only breed for dairy production. From July 1997 to October 1998, blood samples of 1387 cows from five farms (two in the north and three in the south part of Taiwan), and 71 young bulls from 50 farms were collected and DNA extracted for the test. Samples of the young bulls were from north and east part of Taiwan. Six young bulls were detected as carrier (8.5%, 6/71). Ninety-two carriers were found in the five farms (6.6%, 98/1387). The carrier percentages of the five farms were 2.9% (3/103), 5.4% (19/350), 5.5% (7/127), 5.6% (5/89) and 8.1% (58/718) respectively. Total average was 6.7% (98/1458) and no BLAD genotype was found in this investigation. The carrier (BL) percentage of Taiwan was estimated at more than 5%.

並列關鍵字

Dairy cattle ； Bovine leukocyte adhesion deficiency ； Polymerase chain reaction