- 期刊
白羅曼鵝逢機複製多態性DNA片段指印之分析
Polymorphism of Random Amplified Polymorphic DNA in White Roman Geese
摘要
由白羅曼鵝種鵝族群中依公鵝性成熟體重及母鵝產蛋數分成高體重（HBW）、低體重（LBW）、高產蛋（HEP）及低產蛋（LEP）等4組，各組分別有12、12、8及9隻，總共41隻，利用逢機複製多態性DNA（RAPD）方法分析白羅曼鵝DNA多態性。自脛靜脈採集1～3 ml血液，以DNA萃取套組萃取全血之基因組DNA，測定並調整適當濃度後，將所得之DNA作為模板，再以合成之10鹽基對（10 bp）寡核苷酸作為引子，進行聚合酶連鎖反應（polymerase chain reaction, PCR）。PCR複製後之產物以1.5％瓊脂糖膠體進行電泳分析。利用328種不同寡核苷酸序列之逢機引子分析各組鵝隻混合DNA及個體DNA，尋求可能與鵝隻性成熟體重及產蛋數相關之遺傳標記。結果顯示檢測所有供試鵝隻DNA所使用之328種引子中，有85％（279/328）種引子可得到PCR產物，41％（135/328）種引子可檢測出具變異性的片段，在引子AB14（940bp）、AE03（690bp）、AE06（920bp）、AG10（1350bp, 1370bp）、AL13（1288bp）可得具性別特異性的片段；而引子AD06（720bp）與AJ15（490bp）二種引子所產生之特異性DNA片段在高低產蛋數組間之頻率具較大差異。
並列摘要
The purpose of this study was to explore the association between production performance and RAPD (random amplified polymorphic DNA) polymorphism of White Roman Geese. Blood samples of 41 geese were collected based on mature body weight (male) and egg production performance (female). Twelve best or worst geese were assorted into each high body weight (HBW) and low body weight (LBW) groups, while nine best or eight worst geese were assorted into each high egg production (HEP) and Low egg production (LEP) groups. Individual genomic DNA was extracted from blood sample and was detected for ites concentration for DNA template. There were 328 kinds of a single, short (10 bp) oligonucleotide for RAPD tests. After electrophoresis in 1.5% agarose gels and stained with ethidium bromide, the results showed that 85% (279/328) of these primers got PCR products, and 41% (135/328) got polymorphic PCR products. The primers, AB14 (940 bp), AE03 (690 bp), AE06 (920 bp), AG10 (1350 bp , 1370 bp) and AL13 (1288 bp), produced distinct PCR products for different genders and polymorphic patterns of AD06 (720 bp) and AJ15 (490 bp) were not independent between high and low egg production groups.