本研究目的為探討不同接種條件對低、高乾物率的燕麥(29.8%、50.7%)與燕麥/苜蓿混植(33.8%、62.6%)青貯18個月的影響。接種處理包括下列7種:對照(不接種)、處理A(接種Lactobacillus acetotolerans SOR-4,4×10^6 cfu/kg)、處理B(接種Lactobacillus buchneri TNC-5,2×10^8 cfu/kg)、處理C(接種Lactobacillus plantarum L10531,2 × 10^6 cfu/kg)、處理D(接種商業菌劑,L. plantarum, Lactobacillus casei,2 × 10^8 cfu/kg)、處理E(接種商業菌劑+ L. acetotolerans SOR-4)、處理F(接種商業菌劑+ L. buchneri TNC-5)。材料密封於真空袋,每處理4重複,貯放於室內。由變方分析結果,材料、乾物率及接種為青貯發酵重要影響因子,且因子間有交感效應。4批材料的整體表現(pH值、發酵品質、青貯評分)以乾物率33.8%的燕麥/苜蓿表現最佳、依次為乾物率50.7%的燕麥及62.6%的燕麥/苜蓿,乾物率29.8%的燕麥平均表現則為最差。大部分狀況下,接種表現均優於對照。接種處理中以接種L. acetotolerans SOR-4、商業菌劑與同時接種二者的平均表現較佳,而接種L. buchneri TNC-5的表現較差,顯示對燕麥及燕麥/苜蓿的長期保存效果而言,以同質乳酸發酵菌較佳,另L. acetotolerans SOR-4具開發為青貯菌劑的應用潛力。
The purpose of this study aims to investigate the effect of different inoculants on four-set materials ensiling, including oat with dry matter content 29.8% and 50.7%, and oat/alfalfa mixture with dry matter content 33.8% and 62.6%, respectively, for a period of 18 months. The inoculation includes the following 7 treatments: control (no inoculant), Treatment A (inoculated with Lactobacillus acetotolerans SOR-4, 4 × 10^6 cfu/kg), Treatment B (inoculated with Lactobacillus buchneri TNC-5, 2 × 10^8 cfu/kg), Treatment C (inoculated with Lactobacillus plantarum L10531, 2 × 10^6 cfu/kg), Treatment D (inoculated with commercial inoculant, L. plantarum and Lactobacillus casei, 2 × 10^8 cfu/kg), Treatment E (inoculated with commercial inoculant and L. acetotolerans SOR-4) and Treatment F (inoculated with commercial inoculant and L. buchneri TNC-5). The material was treated and sealed in a vacuum bag separately, with four replications per treatment. From the results of variance analysis, material, it showed that dry natter content and inoculation were important factors that affected silage fermentation. In addition, there were interaction effects among these factors. The overall performance (including pH, fermentation quality and Flieg's score) of oat/alfalfa with dry matter content 33.8% was the best among these four-set materials, followed by those of oat with dry matter content 50.7% and oat/alfalfa with dry matter content 62.6%. The overall performance of oat with dry matter content 29.8% showed the worst performance. In most conditions, the fermentation quality of inoculations was better than that of control. However, the treatments inoculated with L. acetotolerans SOR-4, commercial inoculant, and commercial inoculant with L. acetotolerans SOR-4 had better silage quality. On the contrary, the effect inoculated with L. buchneri TNC-5 was poor. The results of this study showed that oats and oats/alfalfa inoculated with homo-fermentative inoculant performed better during the long-period preservation. Further, L. acetotolerans SOR-4 had the potential for developing as the silage additive.