- 期刊
蛋黃免疫球蛋白（IgY）對防治仔豬大腸桿菌下痢之功效探討
Efficacy of egg yolk immunoglobulins (IgY) against Escherichia coli in pigs
摘要
本研究旨在進行抗大腸桿菌之蛋黃免疫球蛋白（immunoglobulin of egg yolk, IgY）對防治仔豬下痢功效的探討。抗大腸桿菌IgY生產是選用市售的四合一疫苗，其中包含大腸桿菌k88、k99、987P、F41等四種纖毛（簡稱為CM抗原），以肌肉注射的方式對來亨蛋雞進行免疫注射。蛋黃中抗大腸桿菌專一性IgY抗體，是以水溶液分離法（water dilution method）來進行分離，並利用抑菌測試及以誘發仔豬下痢模式，進行專一性IgY抗體功效評估。來亨蛋雞經免疫後，所生產抗CM抗原的專一性抗體為82.25μg/mL WSF（water soluble fraction）。在抑菌測試及誘發仔豬下痢防治功效的測試結果顯示，專一性IgY抗體具有抑制大腸桿菌生長，及誘發21天仔豬下痢之保護效果。
並列摘要
The objective of this study was to investigate the immunization response to Escherichia coli (E. coli) antigen of White Leghorn layers and the function of specific immunoglobulin in egg yolk (IgY). The antibody was raised by intramuscular immunization to White Leghorn with inactivated E. coli antigen from the commercial vaccine (k88、k99、987P、F41;CM). Anti-E. coli IgY antibodies were isolated from egg yolk by using water dilution method and their E. coli inhibitory efficiency were determined. The specific IgY from White Leghorn layer immunized with CM antigen had a superior inhibitory effect on the growth of activated E. coli. at a concentration of 82.25μg/mL or more WSF(water soluble fraction). In this study, the chicken egg-yolk antibodies can experimentally prevent diarrhea induced by ETEC in 21 days old weaned piglets.