應用流式細胞儀評估拍賣種公豬精液性狀

Application of flow cytometry to evaluate semen quality traits of auctioned boars

彭麟量(Lin-Liang Peng) ； 林秀蓮(Hsiu-Lien Lin) ； 賴永裕(Yung-Yu Lai) ； 林德育(Der-Yuh Lin) ； 楊鎮榮(Jenn-Rong Yang) ； 許順貴(Shun-Kuei Hsu) ； 劉桂柱(Kuei-Juh Liu) ； 郭廷雍(Ting-Yung Kou)

《畜產研究》 58卷1期 (2025/03) Pp. 22-31

https://doi.org/10.6991/JTLR.202503_58(1).0003

摘要

公豬精液品質直接影響母豬受胎率或產仔數等繁殖性狀，透過公豬精液品質檢測並預測公豬生育力，係維持生產效率避免經濟損失的重要措施。本研究應用流式細胞儀評估參加臺灣區種豬產業協會拍賣之杜洛克與藍瑞斯公豬精液，分析品種、採精日齡、季節等因子對精液性狀的影響，藉以應用至選留產精能力優良種豬。試驗結果顯示藍瑞斯公豬有較高的精液量，但其精液濃度則顯著較杜洛克品種低（P < 0.05）。在採精日齡影響上，超過300日齡的杜洛克公豬在精液量、總精子數、精子存活率、頭帽及精子膜完整性等性狀，均顯著高於240－269日齡與270－299日齡組別公豬（P < 0.05），顯示300日齡以上杜洛克公豬，相較於年輕公豬具較佳的精液性狀；而採精日齡對藍瑞斯公豬則待更多資料累積再進一步分析。在季節影響上，結果顯示季節影響杜洛克及藍瑞斯公豬的精液性狀，其中以秋季採樣精子染色質結構完整性較差。綜上，研究成果可提供未來評估種豬繁殖與選育應用。

關鍵字

精液性狀 ； 流式細胞儀 ； 拍賣種公豬 ； 公豬日齡 ； 採精季節

並列摘要

Semen quality directly affects in the breeding traits such as sow's conception rate or litter size. Boar's semen quality can detect and predict the fertility level of boars and is a key measure in maintaining productive efficiency and avoiding economic losses. This study used flow cytometry to evaluate the semen of Duroc and Landrace boars participating in the Formosan Farmers Association for Swine Improvement auction. The effect of breeds, days of age of semen collection and season on semen traits were analyzed for application and selection of breeding pigs with excellent sperm production ability. The results showed that the Landrace boars had higher semen volume but the semen concentration, compared with that of Landrace boars, was lower (P < 0.05). Regarding the influence of days of age at semen collection, traits such as the semen volume, total sperm counts, sperm viability, and acrosome and sperm membrane integrity of Duroc boars at aged over 300 days were significantly higher than those at age 240 - 269 days and 270 - 299 days (P < 0.05), indicating that Duroc boars over 300 days old have better semen traits, compared with those boars at younger age. While the effect of days of age on Landrace boars requires more data for further analysis. In terms of seasonal effects, the results showed that the chromatin structural integrity is lower in autumn for semen collected from the semen traits of Duroc and Landrace boars. In sum, the research results can be used for future evaluation of breeding and selection in breeding pigs.

並列關鍵字

Semen quality traits ； Flow cytometry ； Auctioned boars ； Boars age ； Semen collection season