- 期刊
稀釋液對臺灣梅花鹿精液冷凍解凍後性狀之影響
Effects of extenders on thawed semen characteristics of Formosan sika deer after freezing
摘要
本研究目的係評估稀釋液種類對臺灣梅花鹿精液冷凍後精液品質之影響。試驗選用健康、性成熟牡臺灣梅花鹿，於10月至12月以電激方式採集精液樣本進行冷凍，分別利用Tris檸檬酸－蛋黃（Tris-citric acid-yolk, TCY）、Tris hydroxymethyl-2-aminomethane-sulphonic acid-蛋黃（TEST-yolk, TY）與檸檬酸－蛋黃（citric acid-yolk, CY）三種稀釋液進行稀釋與冷凍。結果顯示，剛解凍0小時TCY、TY與CY三組之精子活力與前進活力沒有顯著差異，然TCY於平均曲線運動速度顯著優於TY與CY組（P < 0.05），在平均直線運動速度、運動前向性與運動直線性，CY則顯著優於TCY組（P < 0.05）。精液於解凍及培養後進行評估，解凍後1小時各組的精子活力與前進活力沒有顯著差異，然在移動參數評估方面，CY組之平均直線運動速度、運動前向性及運動直線性均顯著優於TCY組（P < 0.05）；解凍後2小時CY組的前進活力優於TCY組（P < 0.05），其餘性狀則各組間無顯著差異。試驗母鹿均經發情同期化處理，於CIDR®移除後60小時進行CY組冷凍解凍精液之人工授精，結果顯示，所有鹿隻整體受胎率為7.1%（1/14）。試驗結果顯示，臺灣梅花鹿精液應用CY稀釋液進行冷凍可得較佳之解凍後活力與精子運動參數。
並列摘要
The purpose of this study was to evaluate the effects of extenders on the semen characteristics of Formosan sika deer after freezing. Healthy and sexually matured male Formosa sika deer were selected for the experiment and the semen collection was conducted by electro-ejaculation between October and December. The semen was diluted with three extenders, including Tris-citric acid-yolk (TCY), Tris hydroxymethyl-2-aminomethane-sulphonic acid-yolk (TEST-yolk; TY) and citric acid-yolk (CY), and then frozen. The results showed that wehre was no significant difference in the sperm motility and progressive motility among the TCY, TY and CY groups. However, the VCL (curvilinear velocity) of TCY group was significantly better than that of TY and CY groups (P < 0.05), and the VSL (straight line velocity), STR (sperm track straightness) and LIN (linearity index) of CY group was significantly better than those of TCY group (P < 0.05). After thawing, the semen was evaluated after thawing and cultivation. There was no significant difference in the sperm motility and progressive motility in 1 hour after thawing. Nonetheless, in terms of the evaluation of progressive parameters, the However, the VSL, STR and LIN of CY group were significantly better than those of TCY group (P < 0.05). The progressive motility of the CY group was significantly better than that of the TCY group in 2 hours after thawing (P < 0.05), and no significant difference was found in the other characteristics among the TCY, TY and CY groups. The doe of the experiment underwent estrus synchronization and performed artificial insemination using semen from CY group after freezing and thawing semen, in 60 hours after CIDR® removal. The results showed that the overall conception rate of all deer was 7.1% (1/14). In conclusion, freezing the semen of Formosan sika deer with CY extender can yield a better motility and progressive characteristics of semen after thawing.