The axolotl is an endangered amphibian which is listed critically endangered species by the international union for conservation of nature (IUCN). Because of its rare characteristic in biology, Axolotl is used in scientific research, how to breed it is worthy to study. The suitable temperature for the axolotl living environment is 14℃to 20℃, but it is too hot in Taiwan. This is a necessary to temperature control of aquarium for the Axolotl. In this article, the water in aquarium is circuited through a thermoelectric chip for cooling. An embedded controller, MyRIO, is used to control the water temperature to the appropriate value for axolotl with pulse width modulation (PWM) technology. As result showing, the water temperature of the aquarium can be maintained at 17±1℃ at an indoor temperature of 27℃ by the proportional-integral feedback control.