The homo-multilayer indium tin oxide (ITO) films have been deposited onto polyethylene terephthalate (PET) substrates by continuous roll-to-roll sputtering at room temperature. While piling up to homo-5-layer ITO film, the best transmittance of 90.12% in visible wavelength and piling up to homo-10-layer ITO film, the sheet resistance of 68Ω/□ are obtained. The TEM diffraction images of ITO films show crystalline at the optimal 5-layer structure, and the nano-sized grains (~50 nm) distributed uniformly all over the surface from TEM investigation. The 5-layer ITO film average roughness is 1.551 nm from AFM investigation.