Review: Beneficial effects of exogenous melatonin on porcine and mouse embryo production in vitro

綜論：外源性添加褪黑激素對於體外培養生成小鼠與豬胚之助益

Jakree Jitjumnong(黃俊淇) ； Pin-Chi Tang(唐品琦)

《中國畜牧學會會誌》 51卷3期 (2022/09) Pp. 261-281

摘要

Melatonin (N-acetyl-5-methoxytryptamine), an indole produced by the pineal gland in animals, is synthesized from tryptophan. The primary role of melatonin in photoperiodic animals is to modify circadian and circannual rhythms. Moreover, melatonin is a free radical scavenger, an antioxidant, and an antiapoptotic substance. Researches have demonstrated that melatonin improves embryo quality by decreasing reactive oxygen species and increasing glutathione production; melatonin also inhibits apoptosis in porcine and mouse embryos. In addition, melatonin is involved in oocyte maturation, fertilization, and embryonic development. The benefits of melatonin on oocyte maturation and embryonic development in vitro have been documented in mammal species, such as mouse, cattle, pig, and human. Since pigs and mice are the most common mammal species used in experimental investigations due to the physiological or genomic resemblance to humans, as well as availability, high reproductive rates, easy handling, and comparatively low cost of usage, this review would address current research on the beneficial effects and roles of melatonin in in vitro production of porcine and mouse oocytes and embryos.

關鍵字

embryo development ； glutathione ； melatonin ； porcine oocytes ； reactive oxygen species

並列摘要

褪黑激素（N-acetyl-5-methoxytryptamine）為動物松果體中由色胺酸合成產生之吲哚類產物，其主要功能為調控動物晝夜與周年之節律，並且褪黑激素亦已知具有清除自由基劑、抗氧化以及抗細胞凋亡之作用。近來研究結果顯示，褪黑激素可經由減少活性氧，增加穀胱甘肽生成而提高體外豬胚胎品質，並抑制其細胞凋亡。此外，褪黑激素亦被證實有益於促進豬、牛、羊、小鼠與人類體外卵母細胞成熟、受精與胚發育過程。因此，本篇綜論將就目前關於褪黑激素對豬隻與小鼠卵母細胞與胚體外生產之助益，以及作用機制之研究進行綜合性闡述。

並列關鍵字

胚發育 ； 穀胱甘肽 ； 褪黑激素 ； 豬隻卵母細胞 ； 活性氧化物質