- 期刊
- OpenAccess
微衛星標記應用於不同褐色菜鴨品系族群遺傳結構之探討
Study on genetic structure in different Brown Tsaiya duck lines with microsatellite markers
摘要
畜產試驗所育有多個褐色菜鴨品系，分別用於保種或選拔，其中選拔品系大多已歷經10代以上長期選拔。為探討目前實施保種或配種策略對於這些族群遺傳歧異度之影響，本試驗利用11組菜鴨微衛星標記，分析4個褐色菜鴨品系之遺傳多態性，並進行同族群跨世代與不同族群間共9個族群之遺傳結構比較。結果顯示褐色菜鴨保種族群之遺傳變異在兩世代幾乎沒有變化，而畜試一號殘差飼料採食量對照組則可能因遺傳漂變造成世代間多態性稍降。不同族群比較部分，結果顯示殘差飼料採食量選拔品系兩世代之有效交替基因數皆低於對照之畜試一號，畜試二號之選拔組及對照組間亦有類似情形。族群結構分析部分，發現保種褐色菜鴨、畜試一號在世代間之分化指數皆小於0.01，顯示目前保種及配種策略可成功維持保種褐色菜鴨及畜試一號之族群遺傳組成。後續將繼續收集更多世代間資料，增加微衛星標記數量持續監控，以確保本土鴨種之遺傳多樣性，並促進種原永續經營利用。
並列摘要
Several Brown Tsaiya duck lines are conserved or selected at the Livestock Research Institute in Taiwan, and most of the selected lines have been selected for over 10 generations. In order to investigate the exact effects of conservative and mating strategies on the genetic diversity of Brown Tsaiya duck populations, we applied the 11 Brown Tsaiya duck-derived microsatellite markers to conduct a genetic analysis to Brown Tsaiya duck lines and made comparison between different generations in a line or different lines. The results showed that genetic variations were almost the same between the two generations in germplasm-preserved Brown Tsaiya ducks, while the diversity of the RFI control line of Brown Tsaiya LRI 1 might be brought down by the genetic drift. On the cross-line comparison, the numbers of effective alleles were higher in control lines than the selected ones in Brown Tsaiya LRI 1 and LRI 2. Among the populations, Brown Tsaiya LRI 2 had comparatively low genetic variations, probably resulting from the smaller population size. The pairwise differentiation indices were less than 0.01 between both of germplasm-preserved Brown Tsaiya duck and the RFI control line of Brown Tsaiya LRI 1. This suggested that the current mating strategies might be helpful to keeping the genetic structure in the two lines. For ensuring diversity of local duck breeds and achieving sustainable production, we need to obtain more information of cross-generation samples and markers to evaluate the genetic status of Brown Tsaiya duck lines in the future.