以偏光方法觀測頭頸癌之膠原蛋白走向特性

Measurement of Collagens in HNSCC by Polarized Light Microscopy

林靖棠(Jing-Tang Lin)

指導教授 ： 董成淵

國立臺灣大學/理學院/應用物理研究所/碩士(2021年)

https://doi.org/10.6342/NTU202103134

摘要

頭頸部鱗狀細胞癌簡稱頭頸癌，為台灣常見癌症之一，肇因酒精以及菸草之大量使用，且已成為台灣嚴峻的健康議題之一。病患經常需要做腫瘤之活體組織切片以此觀察癌症之惡化程度。由於大量證據顯示膠原蛋白纖維之生成可能與腫瘤之轉移及其複製能力有高度相關，本研究以膠原蛋白獨特之雙折射特性研究頭頸癌裡膠原蛋白之走向。我們以瓊斯矩陣(Jones Calculus)運算為基礎構建了一個雙折射理論模型並編寫程式以發展數值計算。實驗裡包含觀察牛筋以及頭頸癌病患之切片，並對19名病患做偏光顯微鏡的全切片影像分析。我們的程式亦表現良好的擬合。

關鍵字

頭頸癌 ； 膠原蛋白 ； 瓊斯矩陣 ； 雙折射 ； 偏光顯微術

並列摘要

Head and neck squamous cell carcinomas (HNSCC) are a type of cancer which are now becoming a serious health issue in Taiwan, due to prevalent alcohol and tobacco use. Patients usually need to take biopsy in order to examine the tumor’s condition. Since there are many evidences showing that the production of collagen fibrils are found related with tumor clones. The collagen’s birefringent patterns, which are distinctive to other tissues, can be applied to investigate in HNSCC studies. Here we implemented the theoretical model of collagen birefringence under the basis of Jones Calculus by scientific programming to research the collagens optical properties in tumors. The slides of beef tendons and HNSCCs are tested in our experiment. We also constructed the polarized microscopy system with Whole Slide Imaging (WSI) to map the distribution of retardance and orientation of birefringence in HNSCC slides for 19 patients. Results of our program tests showed the good quality of the fitting for the orientation and retardance.

並列關鍵字

HNSCC ； Collagen ； Jones Calculus ； Birefringence ； Polarization Microscopy

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