Have library access?
IP:100.26.179.41
  • Theses

薏苡殼與種皮乙醇萃取物對子宮肌瘤細胞增生之抑制效應

Inhibitory Effect of Adlay Hull and Testa Ethanolic Extracts on Uterine Leiomyoma Cell Proliferation

Advisor : 江文章
Co-Advisor : 夏詩閔

Abstracts


No data.

Parallel abstracts


Uterine leiomyoma is becoming a significant health concern affecting one in four women of reproductive age in Taiwan as according to the Ministry of Health and Welfare. The associated symptoms can lead to reduced quality of life and women ultimately seek for surgical intervention. Adlay extract possesses anti-tumor activity and has shown to inhibit sarcoma cell proliferation and tumor progression. In the present study, viability of ELT3 cells was tested to screen for the different fractions of adlay extracts, as well as various phenolic compounds, flavonoids, phytosterols and fatty acids. The active adlay fractions and compounds were then tested on primary human leiomyoma and myometrial smooth muscle cells. Cell cycle profile of ELT3 cells were analyzed in order to determine the possible cause of growth-inhibition. Results showed that AHE-EA, ATE-Hex, and ATE-EA were able to inhibit the proliferation of ELT3 and primary leiomyoma cells. Quercetin, nobiletin, eriodictyol, b-sitosterol, stigmasterol, and stigmastanol were among the tested compounds that significantly decreased the viability of ELT3 and primary leiomyoma cells, while linoleic acid only impeded primary leiomyoma cell growth. Eriodictyol and quercetin induced cell cycle arrest of ELT3 cells at Sub-G1 and G2/M phases. Adlay extract exhibits time and concentration dependent growth-inhibitory effects on uterine leiomyoma cells. These effects may be due to the combined actions of the different compounds, indicating a possibility of a matrix effect from the compounds in the adlay extract.

References


Awad, A.B.; Burr, A.T.; Fink, C.S. Effect of resveratrol and beta-sitosterol in combination on reactive oxygen species and prostaglandin release by PC-3 cells. Prostaglandins Leukot Essent Fatty Acids. 2005, 72, 219-226.
Chan, L.C. 2008. Protective effects of foxtail millet and dehulled adlay on a nonsteroidal anti-inflammatory drug-induced gastric mucosal injury in rats. Master’s Thesis of Graduate Institute of Food Science and Technology of National Taiwan University. Taipei, Taiwan.
Chang, C.H. 2002. Effect of different dehulled adlays on non-specific immune response in BALB/c mice. Master’s Thesis of Graduate Institute of Food Science and Technology of National Taiwan University. Taipei, Taiwan.
Chegini, N. Proinflammatory and profibrotic mediators: principal effectors of leiomyoma development as a fibrotic disorder. Semin Reprod Med. 2010, 28, 180-203.
Chen, Y.J. 2010. The effects on uterine smooth muscle contractions by ethanolic extract of adlay testa. Master’s Thesis of Graduate Institute of Food Science and Technology of National Taiwan University. Taipei, Taiwan.